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Table of Contents
REVIEW ARTICLE
Year : 2022  |  Volume : 21  |  Issue : 2  |  Page : 111-113

Recombinant human chorionic gonadotropin versus purified human chorionic gonadotropin trigger for In vitro Fertilization intracytoplasmic sperm injection cycle


1 Department of Gynaecology and Obstetrics, College of Medicine, Mustansiriyah University, Baghdad, Iraq
2 Khan Dari Primary Health Care Center, Abo Ghraib Health Care Sector, Iraqi Ministry of Health, Baghdad, Iraq

Date of Submission12-Mar-2022
Date of Decision15-May-2022
Date of Acceptance30-May-2022
Date of Web Publication2-Jan-2023

Correspondence Address:
Dr. Zeena Helmi
Department of Gynaecology and Obstetrics, College of Medicine, Mustansiriyah University, Baghdad
Iraq
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/mj.mj_11_22

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  Abstract 

The recombinant human luteinizing hormone or human chorionic gonadotropin (hCG) has substantially taken over the product which was formulated from the urine of pregnant women. In addition to this, a number of randomized controlled trials have been conducted comparing the significance of recombinant hCG (rHCG) with urinary hCG in assisted reproduction. Nevertheless, the present study has collected secondary information based on the nature of the chosen research area. The acquired data and information have been analyzed using content analysis technique to review the methodology and findings of the selected research articles. With the use of rHCG as well as purified hCG, the serum level was equivalent at day 5 among women underwent intracytoplasmic sperm injection (ICSI) but this level abnormally increased among those injected with rHCG thus, minimizing the rate of pregnancy. It has been concluded that purified hCG trigger plays a more efficient role in inducing oocyte maturation and increasing the rate of pregnancy among women undergoing in vitro fertilization ICSI cycle in comparison to rHCG trigger.

Keywords: Assisted reproduction, hCG, trigger


How to cite this article:
Helmi Z, Mussaid Z. Recombinant human chorionic gonadotropin versus purified human chorionic gonadotropin trigger for In vitro Fertilization intracytoplasmic sperm injection cycle. Mustansiriya Med J 2022;21:111-3

How to cite this URL:
Helmi Z, Mussaid Z. Recombinant human chorionic gonadotropin versus purified human chorionic gonadotropin trigger for In vitro Fertilization intracytoplasmic sperm injection cycle. Mustansiriya Med J [serial online] 2022 [cited 2023 Feb 8];21:111-3. Available from: https://www.mmjonweb.org/text.asp?2022/21/2/111/366621


  Introduction Top


For normal conception to occur, luteinizing hormone (LH) surge in the preovulatory phase induces maturation and ovulation. For decades, urinary human chorionic gonadotropin (hCG) has gained significant attention due to its structural similarity with endogenous LH thus achieving final oocyte maturation and an accurate timing to retrieve oocytes in association with protocols of ovarian hyperstimulation in assisted reproduction (in vitro fertilization [IVF] and intracytoplasmic sperm injection [ICSI] cycles).[1] Nevertheless, urinary hCG administration along with multiple follicular developments in cycles leads to the formation of several corpora lutea, as well as, supraphysiological levels of progesterone and estradiol along with sustained luteotrophic effect. Some studies have claimed certain negative effects of hCG on the quality of the embryo and endometrial receptivity despite its use to a greater level, internationally.[2] On the other side, the formation of ovarian hyperstimulation syndrome (OHSS) might be deteriorated and facilitated through the sustained luteotrophic effect. A gonadotrophin-releasing hormone agonist as an alternative to hCG has found to be a role of a trigger in the release of LH, endogenously.[3] The purified gonadotropin has, however, found to be playing a more effective role than recombinant hCG (rHCG) to induce ovulation.

Notwithstanding the fact that purified urinary hCG is observed to be associated with several disadvantages such as constant variation in the activity, uncontrolled source, and lack of purity that could result in a range of different clinical outcomes.[4] In accordance with the World Health Organization, rHCG; as an alternative to the purified hCG, is used with ICSI and IVF for the final oocyte maturation in women. Correspondingly, it has been stated by several other studies that rHCG has substantially taken over the product which was formulated from the urine of pregnant women.[5] In addition to this, a number of randomized controlled trials have been conducted comparing the significance of rHCG with urinary hCG in assisted reproduction. However, equal numbers of oocytes were relatively acquired when women were triggered with either of these treatment groups.

The rhCG is derived through recombinant DNA technology from genetically engineered hamster ovary cells. In comparison to the purified trigger, the rHCG has gained significant attention because of its substantial purity, facilitating the quantitation as well as characterization by means of physicochemical, decreasing the need for bioassays of animals.[5] Nevertheless, little information has been reported to date in the preexisting literature on the cytoplasmic as well as nuclear maturation of women's oocytes acquiring rHCG. As stated by studies that both cytoplasmic and nuclear maturation must be completed in coordination to assure circumstances for consequent fertilization.[6] However, it has been observed that it is quite challenging in standard IVF to evaluate the cytoplasmic morphology of oocytes along with the appropriate phase of maturation due to the fact that cumulus always surrounds the oocytes.[7] The present research has been conducted to determine and assess the comparison between the efficacy of rHCG trigger and purified hCG trigger for IVF-ICSI cycle regarding the number of oocytes retrieved. The secondary objective of this study is to identify the pregnancy rate provided to either of the trigger in the maturation of oocytes.


  Methods Top


Two important online databases have been accessed in this study, including PubMed and ScienceDirect. However, certain filters have been applied on these two databases while retrieving data and information on the efficiency of rHCG trigger and purified urinary hCG trigger for IVF-ICSI cycle. These filters include, the date i.e., the data published since 2010 was accessed, free full-text and both randomized control trials and original research studies were accessed.

To access relevant information on the aforementioned online databases, some key terms have been devised to narrow down the hit of search. These include, “hCG,” “recombinant hCG, “purified hCG,” “recombinant hCG trigger,” “purified hCG trigger,” “ICSI cycle,” and “IVF.”

Research studies were retrieved, and based on their title and abstract, only five of them have been included in the present research to be reviewed and used for the formulation of the research outcomes. The acquired data and information have been analyzed using content analysis technique to review the methodology and findings of the selected research articles.[8],[9]Types of patient participant were those couples with infertility undergoing IVF-ICSI, who had triggering of ovulation with either purified urinary hCG 10000 IU or rHCG 250 μg. The primary outcome was total number of oocytes retrieved, the secondary outcome was live birth rate or pregnancy rate if available.

Inclusion criteria

Eligible studies include those underwent IVF-ICSI with either protocol antagonist or long agonist down regulation, where the age ranges between 20 and 40, and regular menstruation. Studies excluded those who used triggering by gonadotrophin agonist or recombinant LH injections, age more than 40, and cycles of intrauterine insemination or timed intercourse with superovulation.


  Results and Discussion Top


A randomized controlled trial was conducted by Madani et al. reviewed and compared the efficacy of rHCG trigger and purified or urinary hCG trigger in inducing the number of oocytes in ovulation among women who underwent ICSI cycles.[10] Their findings showed evidence that the average number of oocytes retrieved per follicles includes 77.16 ± 17.61, 69.84 ± 17.44, and 71.82 ± 15.09 in 500 μg rHCG, 250 μg rHCG, and 10,000 IU urinary hCG, respectively. They conclude that equivalent number of oocytes was retrieved in both groups.[10]

Similarly, Papanikolaou et al. conducted a randomized control trial of 119 patients with antagonist protocol who claimed that there found to be high number of oocytes retrieved, higher pregnancy rate, and higher live birth rate by rHCG group but similar blastulation rate in both groups, higher first-trimester abortion with the urinary group and higher OHSS with the recombinant group.[11] On the contrary, Srinivas et al. showed evidence that women who were treated with rHCG trigger depicted an equivalent number of oocytes retrieved in both groups but a higher pregnancy rate within the urinary purified group although it is not significant statistically, in addition to similar side effects as OHSS.[12] Berger et al. reviewed the impact of rHCG trigger on the rate of ovulation in the IVF-ICSI cycle, he confirmed similar outcomes of urinary hCG and rHCG.[13]

However, Mosaad et al. showed that the number of oocytes retrieved is similar in both groups but the pregnancy rate was higher in the urinary group. Moreover, rHCG group had higher rate of progesterone elevation[14] and this elevation can have a detrimental effect on implantation and thus on pregnancy rate.[15],[16]

It is evident from these research findings that the effectiveness of purified hCG is significantly higher than rhCG.

Considering the acquired research outcomes, this review highlights increasing the rate of pregnancy among women who undergone an IVF ICSI cycle, purified urinary hCG trigger plays a more efficient role in comparison to recombinant-hCG that reduces the probability of pregnancy in females as a result of its disadvantage in producing abnormal levels of serum progesterone.[17] From the aforementioned results that despite recombinant-hCG trigger being clearer and purer than purified hCG trigger, the number of oocytes produced by both of these treatment groups was equivalent.[10] This finding is against Youssef et al. were they found that the clinical pregnancy rate was similar in both group urinary hCG and rHCG.[18] Another point regarding the number of oocytes retrieved that the number of oocytes was equal in both groups; in line with Eftekhar et al.[1],[18]

This review article has some limitations; it has not provided an in-depth information on the efficiency of other comparative triggers including agonist trigger and recombinant LH, in the context of some other aspects using these two triggers that could play role in enhancing the rate of pregnancy and oocyte maturation in a more effective and pure manner. The strength of this review is that it has significantly determined that the purified hCG trigger is more effective than rHCG trigger. As the number of oocytes retrieved was similar in both groups, but pregnancy rate is higher with the purified could be due to the similarity with the normal physiology of ovulation.


  Conclusion Top


Purified hCG trigger has an equivalent efficacy in inducing oocyte maturation, safety profile, and positive pregnancy rates among women undergoing IVF-ICSI cycle in comparison to rHCG trigger. Since the cost is less with the urinary purified hCG; it should be a recommended first choice for triggering ovulation.

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.

 
  References Top

1.
Eftekhar M, Khalili MA, Rahmani E. The efficacy of recombinant versus urinary HCG in ART outcome. Iran J Reprod Med 2012;10:543-8.  Back to cited text no. 1
    
2.
Lin MH, Wu FS, Lee RK, Li SH, Lin SY, Hwu YM. Dual trigger with combination of gonadotropin-releasing hormone agonist and human chorionic gonadotropin significantly improves the live-birth rate for normal responders in GnRH-antagonist cycles. Fertil Steril 2013;100:1296-302.  Back to cited text no. 2
    
3.
Lin MH, Wu FS, Hwu YM, Lee RK, Li RS, Li SH. Dual trigger with gonadotropin releasing hormone agonist and human chorionic gonadotropin significantly improves live birth rate for women with diminished ovarian reserve. Reprod Biol Endocrinol 2019;17:7.  Back to cited text no. 3
    
4.
Krishna D, Dhoble S, Praneesh G, Rathore S, Upadhaya A, Rao K. Gonadotropin-releasing hormone agonist trigger is a better alternative than human chorionic gonadotropin in PCOS undergoing IVF cycles for an OHSS Free Clinic: A Randomized control trial. J Hum Reprod Sci 2016;9:164-72.  Back to cited text no. 4
[PUBMED]  [Full text]  
5.
Ali SS, Elsenosy E, Sayed GH, Farghaly TA, Youssef AA, Badran E, et al. Dual trigger using recombinant HCG and gonadotropin-releasing hormone agonist improve oocyte maturity and embryo grading for normal responders in GnRH antagonist cycles: Randomized controlled trial. J Gynecol Obstet Hum Reprod 2020;49:101728.  Back to cited text no. 5
    
6.
Pacchiarotti A, Selman H, Valeri C, Napoletano S, Sbracia M, Antonini G, et al. Ovarian stimulation protocol in IVF: An up-to-date review of the literature. Curr Pharm Biotechnol 2016;17:303-15.  Back to cited text no. 6
    
7.
Chen CH, Tzeng CR, Wang PH, Liu WM, Chang HY, Chen HH, et al. Dual triggering with GnRH agonist plus hCG versus triggering with hCG alone for IVF/ICSI outcome in GnRH antagonist cycles: A systematic review and meta-analysis. Arch Gynecol Obstet 2018;298:17-26.  Back to cited text no. 7
    
8.
Bloomfield J, Fisher MJ. Quantitative research design. J Australas Rehabil Nurs Assoc 2019;2:27-30.  Back to cited text no. 8
    
9.
Lowhorn GL. Qualitative and quantitative research: How to choose the best design. In: Academic Business World International Conference. Nashville: Tennessee; 2007.  Back to cited text no. 9
    
10.
Madani T, Mohammadi Yeganeh L, Ezabadi Z, Hasani F, Chehrazi M. Comparing the efficacy of urinary and recombinant hCG on oocyte/follicle ratio to trigger ovulation in women undergoing intracytoplasmic sperm injection cycles: A randomized controlled trial. J Assist Reprod Genet 2013;30:239-45.  Back to cited text no. 10
    
11.
Papanikolaou EG, Fatemi H, Camus M, Kyrou D, Polyzos NP, Humaidan P, et al. Higher birth rate after recombinant hCG triggering compared with urinary-derived hCG in single-blastocyst IVF antagonist cycles: A randomized controlled trial. Fertil Steril 2010;94:2902-4.  Back to cited text no. 11
    
12.
Srinivas MS, Sidhmalswamy GA, Dipika K, Anu K, Mekhala D. Comparing the efficacy of urinary hCG vs. recombinant hCG for final maturation of oocyte in GnRH antagonist IVF/ICSI cycle. Int J Infertil Fetal Med 2012;3:92-6.  Back to cited text no. 12
    
13.
Berger D, Jindal S, McAvey B, Bosler JS, Zapantis A. Should body mass index determine whether a patient receives recombinant or urinary human chorionic gonadotropin for ovulation trigger prior to oocyte retrieval? Fertil Steril 2013;100:s53.  Back to cited text no. 13
    
14.
Mosaad M, Shaban HM, Abd-Allah GM, Rezk GA. Comparison of serum progesterone levels and incidence of pregnancy with urinary and recombinant HCG in women undergoing ICSI. A J Physiol Biochem Pharmacol 2018;8:1-9.  Back to cited text no. 14
    
15.
Nayak S, Ochalski ME, Fu B, Wakim KM, Chu TJ, Dong X, et al. Progesterone level at oocyte retrieval predicts in vitro fertilization success in a short-antagonist protocol: A prospective cohort study. Fertil Steril 2014;101:676-82.  Back to cited text no. 15
    
16.
Helmi Z. Elevation of progesterone in day of trigger in IVF ICSI cycle. World Bull Public Health (WBPH) 2022;6:8-16.  Back to cited text no. 16
    
17.
Lunenfeld B, Bilger W, Longobardi S, Alam V, D'Hooghe T, Sunkara SK. The development of gonadotropins for clinical use in the treatment of infertility. Front Endocrinol (Lausanne) 2019;10:429.  Back to cited text no. 17
    
18.
Youssef MA, Abou-Setta AM, Lam WS. Recombinant versus urinary human chorionic gonadotrophin for final oocyte maturation triggering in IVF and ICSI cycles. Cochrane Database Syst Rev 2016;4:CD003719.  Back to cited text no. 18
    




 

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